KMID : 0043319990220030320
|
|
Archives of Pharmacal Research 1999 Volume.22 No. 3 p.320 ~ p.323
|
|
Anti-allergic Actions of the Leaves of Castnea crenata and Isolation of an Active Component Responsible for the Inhibition of Mast Cell Degranulation
|
|
Lee Eun
Choi Eun-Ju Cheong Ho Kim Young-Ran Ryu Shi-Yong Kim Kyeong-Man
|
|
Abstract
|
|
|
The anti-allergic actions of the leaves of Castanea crenata (Fagaceae) were studied. The water extract demonstrated potent anti-allergic actions in in vivo and in vitro experiments. The oral or intraperitoneal administration of the extract (100 or 200 mg/kg) caused a significant inhibition of the 48 hr-PCA (up to 90%) and the vascular permeability induced by histamine or serotonin in rats (about 80%). The anaphylactic release of -hexosaminidase for RBL-2H3 cells was also significantly inhibited by the extract in as dose-dependent manner with an IC50 value of 230 /ml. The activity-guided fractionation of the extract, based on the determination of inhibitor effect upon the release of -hexosaminidase, led to the isolation of quercetin as an active principle responsible for the inhibition of degranulation.
|
|
KEYWORD
|
|
Allergy, Castanea crenata, Quercetin, 48 hr-PCA, Degranulation
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|