KMID : 0191120180330520336
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Journal of Korean Medical Science 2018 Volume.33 No. 52 p.336 ~ p.336
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Mucosal Immunity Related to FOXP3+ Regulatory T Cells, Th17 Cells and Cytokines in Pediatric Inflammatory Bowel Disease
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Cho Jin-Hee
Kim Sorina Yang Da-Hee Lee Ju-Yeon Park Kyeong-Won Go Jun-Yong Hyun Chang-Lim Jee Young-Heun Kang Ki-Soo
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Abstract
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Background: We aimed to investigate mucosal immunity related to forkhead box P3 (FOXP3+) regulatory T (Treg) cells, T helper 17 (Th17) cells and cytokines in pediatric inflammatory bowel disease (IBD).
Methods: Mucosal tissues from terminal ileum and colon and serum samples were collected from twelve children with IBD and seven control children. Immunohistochemical staining was done using anti-human FOXP3 and anti-ROR¥ãt antibodies. Serum levels of cytokines were analyzed using a multiplex assay covering interleukin (IL)-1¥â, IL-4, IL-6, IL-10, IL-17A/F, IL-21, IL-22, IL-23, IL-25, IL-31, IL-33, interferon (IFN)-¥ã, soluble CD40L, and tumor necrosis factor-¥á.
Results: FOXP3+ Treg cells in the lamina propria (LP) of terminal ileum of patients with Crohn's disease were significantly (P < 0.05) higher than those in the healthy controls. ROR¥ãt+ T cells of terminal ileum tended to be higher in Crohn's disease than those in the control. In the multiplex assay, serum concentrations (pg/mL) of IL-4 (9.6 ¡¾ 1.5 vs. 12.7 ¡¾ 3.0), IL-21 (14.9 ¡¾ 1.5 vs. 26.4 ¡¾ 9.1), IL-33 (14.3 ¡¾ 0.9 vs. 19.1 ¡¾ 5.3), and IFN-¥ã (15.2 ¡¾ 5.9 vs. 50.2 ¡¾ 42.4) were significantly lower in Crohn's disease than those in the control group. However, serum concentration of IL-6 (119.1 ¡¾ 79.6 vs. 52.9 ¡¾ 39.1) was higher in Crohn's disease than that in the control. Serum concentrations of IL-17A (64.2 ¡¾ 17.2 vs. 28.3 ¡¾ 10.0) and IL-22 (37.5 ¡¾ 8.8 vs. 27.2 ¡¾ 3.7) were significantly higher in ulcerative colitis than those in Crohn's disease.
Conclusion: Mucosal immunity analysis showed increased FOXP3+ T reg cells in the LP with Crohn's disease while Th17 cell polarizing and signature cytokines were decreased in the serum samples of Crohn's disease but increased in ulcerative colitis.
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KEYWORD
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Inflammatory Bowel Disease, FOXP3+, Th17, Cytokine
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