KMID : 0191120190340450273
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Journal of Korean Medical Science 2019 Volume.34 No. 45 p.273 ~ p.273
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Isolation of Secretome with Enhanced Antifibrotic Properties from miR-214-Transfected Adipose-Derived Stem Cells
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Park Jung-Hyun
Kim Ok-Hee Kim Kee-Hwan Hong Ha-Eun Seo Hae-Yeon Choi Ho-Joong Ahn Joseph Lee Tae-Yun Kim Say-June
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Abstract
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Background: Secretome refers to the total set of molecules secreted or surface-shed by stem cells. The limitations of stem cell research have led numerous investigators to turn their attention to the use of secretome instead of stem cells. In this study, we intended to reinforce antifibrotic properties of the secretome released from adipose-derived stem cells (ASCs) transfected with miR-214.
Methods: We generated miR-214-transfected ASCs, and extracted the secretome (miR214-secretome) from conditioned media of the transfected ASCs through a series of ultrafiltrations. Subsequently, we intravenously injected the miR-214-secretome into mice with liver fibrosis, and determined the effects of miR-214-secretome on liver fibrosis.
Results: Compared with that by naive secretome, liver fibrosis was ameliorated by intravenous infusion of miR-214-secretome into mice with liver fibrosis, which was demonstrated by significantly lower expression of fibrosis-related markers (alpha-smooth muscle actin, transforming growth factor-¥â, and metalloproteinases-2) in the livers as well as lower fibrotic scores in the special stained livers compared with naive secretome. The infusion of miR-214-secretome also led to lesser local and systemic inflammation, higher expression of an antioxidant enzyme (superoxide dismutase), and higher liver proliferative and synthetic function.
Conclusion: MicroRNA-214 transfection stimulates ASCs to release the secretome with higher antifibrotic and anti-inflammatory properties. miR-214-secretome is thus expected to be one of the prominent ways of overcoming liver fibrosis, if further studies consistently validate its safety and efficiency.
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KEYWORD
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Adipose-Derived Stem Cells, Liver Fibrosis, microRNAs, miR-214, Mesenchymal Stem Cells, Secretome
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