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KMID : 0213520000140010012
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Korean Journal of Ophthalmology
2000 Volume.14 No. 1 p.12 ~ p.19
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Stimulation of macrophage function by S-antigen: production of nitric oxide
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S R Bae
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Abstract
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In this study, we investigated whether retinal soluble proteins, such as S-antigen, play a role in the induction of nitric oxide by macrophages in vitro. Cells from the murine macrophage cell line RAW 264.7 and rat and rabbit peritoneal
macrophages
were
incubated in the presence of retinal soluble protein. The nitrite level in the cultured supernatant was measured to determine nitric oxide production using the Griess reaction. S-antigen induced significant, dose-dependent nitrite production in
both RAW
264.7 and rat peritoneal macrophages. The induction of inducible nitric oxide synthase by retinal protein was inhibited by the iNOS-specific inhibitor, aminoguanidine and the tyrosine inhibitor, genistein. These results show that soluble retinal
protein
significantly induces nitric acid production by macrophages. Increased production of reactive oxygen species by macrophages in the presence of this soluble retinal protein in vivo may accelerate photoreceptor degeneration in uveitis.
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KEYWORD
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