|
KMID : 0213520050190020116
|
|
Korean Journal of Ophthalmology
2005 Volume.19 No. 2 p.116 ~ p.121
|
|
|
Protective Effect of Heat Shock Proteins 70.1 and 70.3 on Retinal Photic Injury after Systemic Hyperthermia
|
|
Kim Jin-Hyoung
Kim Jeong-Hun
Yu Young-Suk
Jeong Seon-Mi
Kim Kyu-Won
|
|
|
Abstract
|
|
|
Purpose: This study aimed to determine the relationship between the heat shock protein 70 from hsps70.1 and 70.3 on retinal photic injury after systemic hyperthermia.
Methods: Eight-week-old female C57BL/6 mice were kept at a constant temperature of 41~42℃ for 25~30 minutes. After dark-adaptation for 8 hours, intense light of 11000 lux was maintained for 6 hours. Histology and immunohistochemistry for the inducible heat shock protein 70 (hsp70), the constitutive heat shock protein 70 (hsc70), and western blot analysis, reverse transcriptase-polymerase chain reaction for hsp70.1 and hsp70.3 were performed just before photic injury and after 1, 4, 7, and 14 days.
Results: Light-induced retinal degeneration was prevented by thermotolerance. After hyperthermia, hsp70 was densely expressed in the inner segment of the photoreceptor layer on the photic injury. Hsp70 expression increased for 4 days after photic injury and slowly decreased thereafter. mRNA from hsp70.3 was induced earlier than that of hsp70.1.
Conclusion: Retinal photic injury was prevented by hyperthermia-induced hsp70. Hsp70 from hsp70.3 may be a rapid and short-lived responder, and that from hsp70.1 is a slower and more sustained responder. Hsp70 from hsp70.3 may be an initial retinal chaperone while hsp70 from hsp70.1 may be a sustained chaperone. Korean Journal of Ophthalmology 19(2):116-121, 2005
|
|
|
KEYWORD
|
|
|
Hsp70.1, Hsp70.3, Hyperthermia, Retinal photic injury
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
   
|
|