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KMID : 0213520070210020111
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Korean Journal of Ophthalmology
2007 Volume.21 No. 2 p.111 ~ p.119
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Microarray for Genes Associated with Signal Transduction in Diabetic OLETF Keratocytes
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Lee Ji-Eun
Lee Jong-Soo
Hwang Sang-Ho
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Abstract
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Purpose: The purpose of this study was to identify differences in signal transduction gene expression between normal and diabetic keratocytes stimulated with interleukin-1α (IL-1α) and tumor necrosis factor-α (TNF-α).
Methods: Normal and diabetic keratocytes were primarily cultured and treated with 20 ng/ml IL-1α and TNF-α for 6 h. cDNA was hybridized to an oligonucleotide microarray. Genes identified by the microarray were further evaluated by real-time PCR.
Results: Diabetic keratocytes over-expressed components of the MAPK and Notch pathways, and under-expressed components of the insulin, calcium, and TGF-β pathways. Cytokine treated diabetic keratocytes differentially expressed components of the TGF-β and MAPK pathways. After IL-1α and TNF-α treatment, nine genes were under-expressed, falling in the insulin, TGF-β, and Toll-like receptor pathways. Real-time PCR showed a significant decrease in the IL-6 and TGF-β2 genes and a significant increase in the Ppm1a gene.
Conclusion: There were some differences in gene expression between normal and diabetic keratocytes related to signal transduction pathways, such as the insulin, MAPK, calcium, and TGF-β pathways. In addition, IL-1α and TNF-α stimulating the insulin, TGF-β, and Toll-like receptor signaling pathways may have different effects in diabetic keratocytes.Korean Journal of Ophthalmology 21(2):111-119, 2007
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KEYWORD
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Diabetes mellitus, Keratocyte, Microarray, Rat, Signaling transduction
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