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KMID : 0213520100240020101
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Korean Journal of Ophthalmology
2010 Volume.24 No. 2 p.101 ~ p.107
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Protective Effect of Catechin on Apoptosis of the Lens Epithelium in Rats with N-methyl-N-nitrosourea-induced Cataracts
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Lee Sung-Min
Ko II-Gyu
Kim Sung-Eun
Kim Dong-Hee
Kang Byung-Nam
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Abstract
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Purpose: To investigate the effect of catechin on apoptotic cell death in the lens epithelium of rats with cataract.
Methods: Cataract was induced by intraperitoneal injection of 100 mg/kg N-methyl-N-nitrosourea (MNU) to ten day-old Sprague-Dawley rats. The neonatal rats were randomly divided into five groups (n=15 in each group): a control group, and four cataract-induction groups, treated with either 0, 50, 100, 200 mg/kg catechin. We performed slit-lamp biomicroscopic analysis, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay, Western-blot for Bcl-2 and Bax, and immunohistochemistry for caspase-3.
Results: Apoptotic cell death in lens epithelial cells that increased following cataract formation in rats was suppressed by cathechin.
Conclusions: Catechin inhibited cataract-induced apoptotic cell death in the lens epithelium and may prove useful for the prevention of cataract progression.
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KEYWORD
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Apoptosis, Cataract, Catechin, Crystalline lens, Epithelial cells, Rats
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