KMID : 0213520100240030169
|
|
Korean Journal of Ophthalmology 2010 Volume.24 No. 3 p.169 ~ p.172
|
|
The Effect of Brimonidine on Transepithelial Resistance in a Human Retinal Pigment Epithelial Cell Line
|
|
Park Jung-Hyun
Kim Sung-Joon Yu Hyeong-Gon
|
|
Abstract
|
|
|
Purpose: To investigate the effects of brimonidine, an ¥á-2-adrenergic agonist, on barrier function in ARPE-19 cells by measuring transepithelial resistance (TER).
Methods: ARPE-19 cells were cultured into a confluent monolayer on a microporous filter. Brimonidine was added to the apical medium, and the barrier function of the cells was evaluated by measuring TER. A subset of cells was treated under hypoxic conditions, and the TER changes observed upon administration of brimonidine were compared to those observed in cells in normoxic conditions.
Results: The ARPE cell membrane reached a peak resistance of 29.1¡¾7.97 ¥Øcm2 after four weeks of culture. The TER of the cells treated under normoxic conditions increased with brimonidine treatment; however, the TER of the cells treated under hypoxic conditions did not change following the administration of brimonidine.
Conclusions: Barrier function in ARPE-19 cells increased with brimonidine treatment. Understanding the exact mechanism of this barrier function change requires further investigation.
|
|
KEYWORD
|
|
Adrenergic agonist, Blood-retinal barrier, Brimonidine, Retinal pigment epithelium
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|