KMID : 0213520150290060424
|
|
Korean Journal of Ophthalmology 2015 Volume.29 No. 6 p.424 ~ p.432
|
|
Effects of Bevacizumab on Bcl-2 Expression and Apoptosis in Retinal Pigment Epithelial Cells under Oxidative Stress
|
|
Kim Suk-Jin
Kim Young-Jun Kim Na-Rae Chin Hee-Seung
|
|
Abstract
|
|
|
Purpose: To evaluate the effects of bevacizumab on expression of B-cell leukemia/lymphoma (Bcl)-2 and apoptosis in retinal pigment epithelial (RPE) cells under oxidative stress conditions.
Methods: RPE cells were treated with H2O2 (0, 100, 200, 300, and 400 ¥ìM) and bevacizumab at or above the doses normally used in clinical practice (0, 0.33, 0.67, 1.33, and 2.67 mg/mL). Cell apoptosis was measured using flow cytometry with annexin V-fluorescein isothiocyanate. The expression of Bcl-2 mRNA was determined using reverse transcription polymerase chain reaction.
Results: Under low oxidative stress conditions (H2O2 100 ¥ìM), cell apoptosis was not significantly different at any concentration of bevacizumab, but Bcl-2 mRNA expression decreased with increasing concentration of bevacizumab (0.33, 0.67, 1.33, and 2.67 mg/mL). Under moderate oxidative stress conditions (H2O2 200 ¥ìM), Bcl-2 mRNA expression decreased with increasing concentration of bevacizumab (0.33, 0.67, 1.33, and 2.67 mg/mL), but cell apoptosis increased only at 2.67 mg/mL of bevacizumab. Under high oxidative stress (300 ¥ìM) conditions, cell apoptosis increased at high concentrations of bevacizumab (1.33 and 2.67 mg/mL), but it did not correlate with Bcl-2 expression.
Conclusions: Withdrawal of vascular endothelial growth factor can lead to RPE cell apoptosis and influences the expression of anti-apoptotic genes such as Bcl-2 under oxidative stress conditions. Since oxidative stress levels of each patient are unknown, repeated injections of intravitreal bevacizumab, as in eyes with age-related macular degeneration, might influence RPE cell survival.
|
|
KEYWORD
|
|
Apoptosis, Bcl-2, Bevacizumab, Retinal pigment epithelial cell
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|