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KMID : 0311119780190010070
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Yonsei Medical Journal
1978 Volume.19 No. 1 p.70 ~ p.74
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Modified Dextrose Starch Agar for the Preservation of Neisseria gonorrhoeae
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Chong, Yunsop
Kim, Sung Ok/Lee, Samuel Y.
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Abstract
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To seek a practical and inexpensive method to preserve gonococcal cultures, a few methods were compared. The following methods kept the cultures alive for only a short period of time: skim milk at -20℃; tryptic soy broth with 15% glycerol at -20℃; cystine tryptic agar at 35℃. Most of the test cultures survived for more than 4 weeks in the following media: one half strength dextrose starch agar; one half strength dextrose starch agar with ferric nitrate; one half strength dextrose starch agar with antimicrobic CNV. Dextrose starch agar could be substituted by GC medium base with a slight modification. It is concluded that preservation of gonococci in one half strength dextrose starch agar with CNV, which produces less frequent contamination, is a practical method to maintain cultures for teaching and quality control.
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