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KMID : 0311119800210010018
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Yonsei Medical Journal
1980 Volume.21 No. 1 p.18 ~ p.23
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Effect of Dexamethasone and Epinephrine on Metallothionein Level in the Perfused Rat Liver
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Lee Hae-Soon
Oh Sang-Hwan
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Abstract
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The effect of dexamethasone (10^(-5)M) and epinephrine (10^(-6)M) on the biosynthesis of metallothionein (MT) in the perfused rat liver was investigated. MT synthesis was determined by measuring the incorporation of ^(14)C-L-aspartic acid into liver MT fraction after the perfusion for five hours of isolated liver by artificial blood containing ^(14)C-L-U-aspartic acid (0.2uci) with dexamethosone or epinephrine.
MT was isolated by Sephadex G-75 column chromatography and DEAE Sephadex column chromatography. Incorporation of radioactive ^(14)C into the MT fraction of perfused liver cytosol (9.Ograms of liver) from dexamethasone treated, epinephrine treated and control groups were, respectively, 0.72, 0.34 and 0.33% of total radioactivity infused. Total protein content in the MT fraction of liver perfused with dexamethasone and epinephrine were 0. 80, 0.64㎎/g liver compared to 0.52㎎/g liver in
the control. MT, a protein having a high content of cystein and metals is synthesized in the perfused rat liver and its induction is stimulated by dexamethasone, while epinephrine increased the accumulation of Zn in the MT fraction of the perfused rat liver.
The present experiment confirms that MT synthesis and degradation are somewhat regulated by glucocorticoid hormone and epinephrine.
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