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KMID : 0311119990400030283
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Yonsei Medical Journal
1999 Volume.40 No. 3 p.283 ~ p.289
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Identification and Purification of IgE-reactive Proteins in German Cockroach Extract
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Tai Soon Yong
Jong Seok Lee/Jongweon Lee/Soon Jung Park/Soung Hoo Jeon/Han Il Ree/Jung Won Park
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Abstract
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Cockroaches have been implicated as a cause of respiratory allergy in urban areas worldwide. IgE-reactive German cockroach proteins were identified with molecular weights (MWs) of 90, 66, 50, 43 and 36 KD by immunoblot analysis in both immune BALB/c mice and sensitized humans. Prominent IgE-reactive proteins were purified using FPLC by ion-exchange chromatography, gel filtration and hydrophobic chromatography. The N-terminal amino acid sequence of a purified protein with a MW of 66 KD on SDS-PAGE was Val-Thr-Leu-Lys-Lys(Val)-Met-Ile-Lys-Thr-Phe-Tyr. No homologous protein was found through a search of GenBank that indicated a novel IgE-reactive protein in German cockroach extract. Another purified protein with a MW of 36 KD reacted strongly with a monoclonal antibody against Bla g 2.
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KEYWORD
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German cockroach, IgE, allergens, immunoblot, amino acid sequence,
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