|
KMID : 0311119990400050490
|
|
Yonsei Medical Journal
1999 Volume.40 No. 5 p.490 ~ p.495
|
|
|
Regulation of Type I Collagen and Interstitial Collagenase mRNA Expression in Human Dermal Fibroblasts by Colchicine and D-penicillamine
|
|
Kee Yang Chung
Dong Seung Kang
|
|
|
Abstract
|
|
|
|
Sclerosis is a disease process in which idiopathic hardening occurs in the skin and/or internal organs as a result of the accumulation of type I collagen, induced mainly by transforming growth factor-β. Colchicine and D-penicillamine are widely used for its treatment. Their effects are known to be due to post-translational down-regulation of type I collagen synthesis, with colchicine also up-regulating interstitial collagenase. To determine whether or not they have any pre-translational effect on type I collagen and MMP-1, and also to observe their effects on the action of TGF-β, cultured neonatal foreskin fibroblasts were treated with colchicine and D-penicillamine, singly and together. The amount of type I collagen and MMP-1 mRNA were quantitated by Northern blot hybridization. Colchicine suppresses the basal level of type I collagen mRNA but minimally stimulates the mRNA expression of MMP-1, whereas D-penicillamine does not have any significant effects on either. Colchicine was also able to significantly suppress the TGF-β-induced up-regulation of type I collagen mRNA expression.
|
|
|
KEYWORD
|
|
|
Sclerosis, type I collagen, interstitial collagenase, mRNA, TGF-β, colchicine, D-penicillamine,
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
   
|