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KMID : 0311120070480040684
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Yonsei Medical Journal
2007 Volume.48 No. 4 p.684 ~ p.693
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Heterogeneous Composition of Voltage-Dependent K+ Currents in Hepatic Stellate Cells
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Lee Dong-Hyeon
Kim Kuc-Han
Kim Hyung-Lae
Han Bok-Ghee
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Abstract
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Purpose:Hepatic stellate cells (HSC) are a type of pericyte with varying characteristics according to their location. However, the electrophysiological properties of HSC are not completely understood. Therefore, this study investigated the difference in the voltage-dependent K+rrents in HSC.
Materials and Methods:The voltage-dependent K+ currents in rat HSC were evaluated using the whole cell configuration of the patch-clamp technique.
Results:Four different types of voltage-dependent K+ currents in HSC were identified based on the outward and inward K+ currents. Type D had the dominant delayed rectifier K+ current, and type A had the dominant transient outward K+ current. Type I had an inwardly rectifying K+ current, whereas the non-type I did not. TEA (5mM) and 4-AP (2mM) suppressed the outward K+currents differentially in type D and A. Changing the holding potential from -80 to -40mV reduced the amplitude of the transient outward K+ currents in type A. The inwardly rectifying K+ currents either declined markedly or were sustained in type I during the hyperpolarizing step pulses from -120 to -150mV.
Conclusion:There are four different configurations of voltage-dependent K+ currents expressed in cultured HSC. These results are expected to provide information that will help determine the properties of the K+ currents in HSC as well as the different type HSC populations.
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KEYWORD
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Hepatic stellate cells, voltage-dependent K+ currents, different cell population
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