KMID : 0311120080490020230
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Yonsei Medical Journal 2008 Volume.49 No. 2 p.230 ~ p.236
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The Effect of Chlamydia pneumoniae on the Expression of Peroxisome Proliferator-Activated Receptor-¥ã in Vascular Smooth Muscle Cells
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Kim Yong-Hwan
Seung Ki-Bae Wang Young-Pil Chang Ki-Yuk Choi Si-Young Suh Jong-Hui Kim Tae-Kyun
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Abstract
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Purpose: This study was designed to investigate the change of peroxisome proliferator-activated receptor gamma (PPAR¥ã) after the infection of the human coronary artery smooth muscle cells (HCSMCs) with Chlamydia pneumoniae (C. pneumoniae) and the effect of PPAR¥ã agonist on the expression of PPAR¥ã of C. pneumoniae-infected HCSMCs.
Materials and Methods: To determine the effect of PPAR¥ã agonist on the proliferation of C. pneumoniae-infected HCSMCs, rosiglitazone at various concentrations was applied 1 hour before inoculation of HCSMCs.
Results: The expression of PPAR¥ã mRNA in HCSMCs increased from 3 hours after C. pneumoniae infection and reached that of noninfected HCSMCs at 24 hours (p<0.05). The expression of PPAR¥ã protein in HCSMCs also increased from 3 hours after C. pneumoniae and persisted until 24 hours as compared with that of noninfected HCSMCs (p<0.05). The pretreatment of HCSMCs with rosiglitazone followed by the infection with C. pneumoniae augmented the expression of PPAR¥ã mRNA and protein (p<0.05) and decreased cell proliferation.
Conclusion: Our results showed that the expression of PPAR¥ã increases in response to C. pneumoniae infection and rosiglitazone further augmented the expression of PPAR¥ã. It is suggested that rosiglitazone could ameliorate the chronic inflammation in the vessel wall induced by C. pneumoniae by augmenting PPAR¥ã expression.
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KEYWORD
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C, pneumoniae, peroxisome proliferator-activated receptor gamma, rosiglitazone, atherosclerosis
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