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KMID : 0311120080490020301
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Yonsei Medical Journal
2008 Volume.49 No. 2 p.301 ~ p.310
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Identification of Up-Regulated Genes in Malignant Glioma with Subtraction Hybridization: Preliminary Screening Studies
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Cho Yong-Jae
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Abstract
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Purpose: This investigation is intended to obtain differentially expressed genes related to human malignant glioma using Subtractive hybridization.
Materials and Methods: Subtractive hybridization is potentially faster methods for identifying differentially expressed genes associated with a particular disease state. We identified 7 over-expressed genes which were not homologous to any of the known genes in the GenbankTM database.
Results: Using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), the mRNA expression levels of these 7 genes were higher in human glioblastomas tissue than in non-tumor brain tissue. In order to learn more about the expression profile of these genes, RT-PCR was performed using various commercially available human carcinoma cell lines. Some of these new genes were over-expressed in human glioma cell line, but not the expressed in other human cancer cell line.
Conclusion: Theses cloned new genes may play a role in brain tumorigenesis. Further studies including verification of oncogene, cancer protein, and glioblastoma induction in animal model are needed.
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KEYWORD
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Glioblastoma, gene, subtraction hybridization
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