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KMID : 0311120080490030459
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Yonsei Medical Journal
2008 Volume.49 No. 3 p.459 ~ p.471
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Changes in Inward Rectifier K+ Channels in Hepatic Stellate Cells During Primary Culture
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Lee Dong-Hyeon
Park Kyu-Sang
Lee Joong-Woo
Kong In-Deok
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Abstract
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Purpose: This study examined the expression and function of inward rectifier K+ channels in cultured rat hepatic stellate cells (HSC).
Materials and Methods: The expression of inward rectifier K+ channels was measured using real-time RT-PCR, and electrophysiological properties were determined using the gramicidin-perforated patch-clamp technique.
Results: The dominant inward rectifier K+ channel subtypes were Kir2.1 and Kir6.1. These dominant K+ channel subtypes decreased significantly during the primary culture throughout activation process. HSC can be classified into two subgroups: one with an inward-rectifying K+ current (type 1) and the other without (type 2). The inward current was blocked by Ba2+ (100μM) and enhanced by high K+ (140mM), more prominently in type 1 HSC. There was a correlation between the amplitude of the Ba2+-sensitive current and the membrane potential. In addition, Ba2+ (300μM) depolarized the membrane potential. After the culture period, the amplitude of the inward current decreased and the membrane potential became depolarized.
Conclusion: HSC express inward rectifier K+ channels, which physiologically regulate membrane potential and decrease during the activation process. These results will potentially help determine properties of the inward rectifier K+ channels in HSC as well as their roles in the activation process.
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KEYWORD
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Hepatic stellate cells, inward rectifier K+ channels, electrophysiology, real-time RT-PCR
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