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KMID : 0311120090500010060
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Yonsei Medical Journal
2009 Volume.50 No. 1 p.60 ~ p.67
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Identification of Differentially Expressed Genes in Papillary Thyroid Cancers
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Lee Ki-Young
Kim Jin-Man
Huang Song-Mei
Li Sheng-Jin
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Abstract
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Purpose: Techniques designed to identify differentially expressed genes (DEGs) in tumors have become important in modern pathology. Genefishing techniqueTM using the annealing control primer (ACP) system has recently been developed to screen for DEG transcripts. We tried to identify DEGs involved in papillary thyroid cancer (PTC) by using Genefishing techniqueTM.
Materials and Methods: We utilized a new differential display method, designated with Genefishing techniqueTM, to analyze DEGs in 21 cases of PTCs.
Results: Comparing the gene expression profiles between PTC and normal thyroid, we detected 17 genes that were differentially expressed in PTCs and performed cloning with sequencing in 10 genes. We confirmed the expression patterns of 2 DEGs by RT-PCR assay and identified the same results in 17 out of 21 (81%) PTCs. The 2 DEGs over-expressed in PTCs were identified as DC-STAMP and type I collagen A1. They are novel genes identified first in PTCs.
Conclusion: We confirmed 2 DEGs in PTCs as DC-STAMP and type I collagen A1 by using Genefishing techniqueTM. Although the detailed functions of those 2 genes and their products remain to be determined, the genes will provide insights into mechanisms of carcinogenesis or tumor progression in PTCs.
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KEYWORD
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Papillary thyroid cancer, differentially expressed genes, dendritic cell-specific transmembrane protein, type I collagen A1
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