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KMID : 0311120110520060999
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Yonsei Medical Journal
2011 Volume.52 No. 6 p.999 ~ p.1007
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Rapid Isolation of Adipose Tissue-Derived Stem Cells by the Storage of Lipoaspirates
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Eom Young-Woo
Lee Jong-Eun
Yang Mal-Sook
Jang In-Keun
Kim Hyo-Eun
Lee Doo-Hoon
Kim Young-Jin
Park Won-Jin
Kong Jee-Hyun
Shim Kwang-Yong
Lee Jong-In
Kim Hyun-Soo
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Abstract
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Purpose: This study examined a rapid isolation method decreasing the time and cost of the clinical application of adipose tissue-derived stem cells (ASCs).
Materials and Methods : Aliquots (10 g) of the lipoaspirates were stored at 4℃ without supplying oxygen or nutrients. At the indicated time points, the yield of mononuclear cells was evaluated and the stem cell population was counted by colony forming unit-fibroblast assays. Cell surface markers, stem cell-related transcription factors, and differentiation potentials of ASCs were analyzed.
Results: When the lipoaspirates were stored at 4℃, the total yield of mononuclear cells decreased, but the stem cell population was enriched. These ASCs expressed CD44, CD73, CD90, CD105, and HLA-ABC but not CD14, CD31, CD34, CD45, CD117, CD133, and HLA-DR. The number of ASCs increased 1×1014 fold for 120 days. ASCs differentiated into osteoblasts, adipocytes, muscle cells, or neuronal cells.
Conclusion: ASCs isolated from lipoaspirates and stored for 24 hours at 4℃ have similar properties to ASCs isolated from fresh lipoaspirates. Our results suggest that ASCs can be isolated with high frequency by optimal storage at 4℃ for 24 hours, and those ASCs are highly proliferative and multipotent, similar to ASCs isolated from fresh lipoaspirates. These ASCs can be useful for clinical application because they are time- and cost-efficient, and these cells maintain their stemness for a long time, like ASCs isolated from fresh lipoaspirates.
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KEYWORD
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Lipoaspirates, adipose tissue, mesenchymal stem cell, proliferation, differentiation
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