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KMID : 0311120180590050633
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Yonsei Medical Journal
2018 Volume.59 No. 5 p.633 ~ p.642
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Effects of the Helicobacter pylori Virulence Factor CagA and Ammonium Ion on Mucins in AGS Cells
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Zhang Xiaoyu
Shi Ding
Liu Yong-Pan
Chen Wu-Jie
Wu Dong
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Abstract
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Purpose: To investigate the effects of Helicobacter pylori (H. pylori)-CagA and the urease metabolite NH4+ on mucin expression in AGS cells.
Materials and Methods: AGS cells were transfected with CagA and/or treated with different concentrations of NH4CL. Mucin gene and protein expression was assessed by qPCR and immunofluorescence assays, respectively.
Results: CagA significantly upregulated MUC5AC, MUC2, and MUC5B expression in AGS cells, but did not affect E-cadherin and MUC6 expression. MUC5AC, MUC6, and MUC2 expression in AGS cells increased with increasing NH4+ concentrations until reaching a peak level at 15 mM. MUC5B mRNA expression in AGS cells (NH4+ concentration of 15 mM) was significantly higher than that at 0, 5, and 10 mM NH4+. No changes in E-cadherin expression in AGS cells treated with NH4+ were noted, except at 20 mM. The expression of MUC5AC, MUC2, and MUC6 mRNA in CagA-transfected AGS cells at an NH4+ concentration of 15 mM was significantly higher than that at 0 mM, and decreased at higher concentrations. The expression of MUC5B mRNA increased with increases in NH4+ concentration, and was significantly higher compared to that in untreated cells. No significant change in the expression of E-cadherin mRNA in CagA-transfected AGS cells was observed. Immunofluorescence assays confirmed the observed changes.
Conclusion: H. pylori may affect the expression of MUC5AC, MUC2, MUC5B, and MUC6 in AGS cells via CagA and/or NH+4, but not E-cadherin.
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KEYWORD
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Mucin, Helicobacter pylori, CagA, ammonium chloride, stomach
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