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KMID : 0311120220630070632
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Yonsei Medical Journal
2022 Volume.63 No. 7 p.632 ~ p.639
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Effects of Interleukin-17A on the Early Stages of Arterial Thrombosis in Mice
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Park Young-Seon
Shim Ye-Seul
Kwon Il
Lee Heow-Won
Nam Hyo-Suk
Choi Hyun-Jung
Heo Ji-Hoe
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Abstract
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Purpose: Interleukin (IL)-17A has been suggested to play a role in the growth and organization of thrombi. We examined whetherIL-17A plays a role in the early stages of thrombosis and whether there are sex differences in the effects of IL-17A.
Materials and Methods: We performed a blinded, randomized, placebo-controlled study to compare time to thrombotic occlu sion and sex differences therein between mice treated with IL-17A and those treated with saline using a ferric chloride-inducedmodel. We also assessed thrombus histology, blood coagulation, and plasma levels of coagulation factors.
Results: Time to occlusion values did not differ between the IL-17A group and the control group (94.6±86.9 sec vs. 121.0±84.4 sec,p=0.238). However, it was significantly shorter in the IL-17A group of female mice (74.6±57.2 sec vs. 130.0±76.2 sec, p=0.032). Inrotational thromboelastometry, the IL-17A group exhibited increased maximum clot firmness (71.3±4.5 mm vs. 66.7±4.7 mm,p=0.038) and greater amplitude at 30 min (69.7±5.2 mm vs. 64.5±5.3 mm, p=0.040) than the control group. In Western blotting,the IL-17A group showed higher levels of coagulation factor XIII (2.2±1.5 vs. 1.0±0.9, p=0.008), monocyte chemoattractant pro tein-1 (1.6±0.6 vs. 1.0±0.4, p=0.023), and tissue factor (1.5±0.6 vs. 1.0±0.5, p=0.003).
Conclusion: IL-17A plays a role in the initial st ages of arterial thrombosis in mice. Coagulation factors and monocyte chemoat tractant protein-1 may be associated with IL-17A-mediated thrombosis.
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KEYWORD
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Thrombosis, interleukin-17A, inflammation, monocyte chemoattractant protein-1, tissue factor, factor XIII
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