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KMID : 0311120230640030167
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Yonsei Medical Journal
2023 Volume.64 No. 3 p.167 ~ p.174
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Effects of Mesenchymal Stem Cells Treatment on Radiation-Induced Proctitis in Rats
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Kim Won-Hee
Yoo Jun-Hwan
Yoo In-Kyung
Kwon Chang-Il
Hong Sung-Pyo
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Abstract
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Purpose: There are no effective treatment methods with which to control complications of radiation proctitis with fistula or re current bleeding following radiation treatment for prostate, cervical, or rectal cancer. Mesenchymal stem cells (MSCs) can induce immune modification, resulting in tissue repair and regeneration. Therefore, we used a rat model of radiation-induced proctitis and observed the effects of using human placenta-derived (PD) and adipose tissue-derived (AD) MSCs.
Materials and Methods: Female Sprague Dawley rats were irradiated at the pelvic area with 25 Gy. We injected 1×106 cells of hu man PD-MSCs, human AD-MSCs, human foreskin fibroblasts, and control media into the rectal submucosa following irradia tion. We sacrificed rats for pathologic evaluation.
Results: Fibrosis on the rectum was reduced in both MSC groups, compared to the control group. Mucosal Ki-67 indices of both MSC injected groups were higher than those in the control group. Although caspase-3 positive cells in the mucosa gradually in creased and decreased in the control group, those in both MSC injected groups increased rapidly and decreased thereafter.
Conclusion: We demonstrated the effects of regional MSC injection treatment for radiation-induced proctitis in rats. MSC injec tion reduced fibrosis and increased proliferation in rat mucosa. Human AD-MSCs and PD-MSCs had similar effectiveness.
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KEYWORD
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Proctitis, radiotherapy, mesenchymal stem cells, rat model
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