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KMID : 0311219760070010026
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Yonsei Reports on Tropical Medicine
1976 Volume.7 No. 1 p.26 ~ p.39
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Indirect Fluorescent Antibody Test for the Serodiagnosis of Paragonimiasis and Clonorchiasis
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Cho Kee-Mok
Soh Chin-Thack
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Abstract
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The indirect fluorescent antibody(IFA) test was developed for the serodiagnosis of paragcnimiasis and clonorchiasis by using delipidized adult Paragonimus westermani and Clonorchis sinensis particles, fixed cn microscope slides, as antigens.
The usefulness of this technique was assessed with 6 batches of human sera: 25 sera from paragcnimiasis patients, 38 sera from clonorchiasis patients, 68 sera from persons with parasitic infections other than Paragonimus zestermani and Clonorchis sinensis, 18 sera from non-parasitic lung diseases, 12 sera from non-parasitic liver diseases, and 20 sera it-cm healthy individuals. Additionally, 8 sera from persons of paragonimiasis were used to examine the changes of IFA reactivity after the complete elimination of the parasite, and 31 dried blood smears cn filter paper were tested for comparison of IFA titers with serum samples. Absorption experiment was also performed to test specificity of the antigens.
In the IFA test for paragonimiasis, all serum samples from paragonimiasis patients were correctly diagnosed and false positives were only 1.2% among sera from persons with parasitic infections other than P. westermani. In the IFA test for clonorchiasis 94.7% of the sera from clonorchiasis patients was diagnosed as positive and 5.3% showed doubtful reaction. Among sera from persons with parasitic infections other than C.sinensis, 94.6% was diagnosed as negative and 5.4% showed doubtful reaction. In both tests, all specimens from non-parasitic diseases and healthy controls showed negative reaction.
Cross reaction, with titers of 1 : 8 or less, occurred occasionally between Paragonimus and Clonorchis antigens, but homologous antigen showed always 2?7 fold higher
titers. In absorption experiment, homolo gous reactivity was completely removed. All the cases of cross reaction and a titer cf 1: 8 in ~crun! foam cured paragcnimiasis case were absorbed by both homcicgevs and heterclegous antigen. In cured paragcnimiasis cases, IFA test revealed either negative or doubtful reactions. Ccml,ariscn cf IFA titers between serum samples and dried blood specimens cn filter paper showed a highly significant correlation.
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