KMID : 0338420010160030180
|
|
The Korean Journal of Internal Medicine 2001 Volume.16 No. 3 p.180 ~ p.186
|
|
Effects of BCG infection on Schultz-Dale reaction, allergen-specific IgE levels, and Th2 immune response in sensitized rats
|
|
Inseon S Choi/Youngil I Koh
Inseon S Choi/Won Young Kim/Hyun Chul Lee*/Jon GunLee¢Ó
|
|
Abstract
|
|
|
Background : BCG, a potent inducer of Th1 immune response, has been suggested to suppress Th2 response which is known to mediate IgE-mediated allergic disorders, in particular allergic asthma. Schultz-Dale reaction is known to be a model of IgE-mediated hypersensitivity. This study was done to investigate whether BCG infection suppresses the Schultz-Dale reaction by inhibiting Th2 response and allergen-specific IgE production.
Methods : Twenty-four Sprague-Dawley rats were sensitized and provoked with ovalbumin (OVA). A pretreatment of 6¡¿104 colony forming units of BCG or saline was done 7 days before sensitization. The Schultz-Dale reaction was represented as tracheal smooth muscle contractions to 50 ¥ìg/mL OVA challenge in vitro. Serum OVA-specific IgE levels and IFN-¥ã and IL-4 concentrations in bronchoalveolar lavage fluid (BALF) were measured.
Results : The Schultz-Dale reaction and serum OVA-specific IgE levels were significantly decreased in BCG infected and OVA sensitized rats compared with only sensitized rats (p<0.0l and p<0.05, respectively). As compared with only sensitized rats, IL-4 concentration and a ratio of IFN-¥ã : IL-4 in BCG infected and OVA sensitized rats were significantly decreased (p<0.001) and increased (p<0.05), respectively. The Schultz-Dale reaction was correlated with OVA-specific IgE levels (r=0.50, p<0.05), IL-4 concentration (r=0.69, p<0.001), and ratio of IFN- : IL-4 (r=?0.44, p<0.05). OVA-specific IgE levels were correlated with IL-4 concentration (r=0.61, p<0.01) and ratio of IFN-¥ã : IL-4 (r=?0.48, p<0.05).
Conclusion : These findings suggest that BCG infection prior to allergen sensitization may inhibit Schultz-Dale reaction developed in the sensitized rat tracheal smooth muscle via the suppressive effects of Th2 immune response and allergen-specific IgE production.
|
|
KEYWORD
|
|
BCG vaccine, Asthma, Schultz-Dale reaction, Cytokines,
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|