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KMID : 0338420080230040165
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The Korean Journal of Internal Medicine
2008 Volume.23 No. 4 p.165 ~ p.169
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Hepatocyte nuclear factor 1-alpha mutation in normal glucose-tolerant subjects and early-onset type 2 diabetic patients
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Lim Dong-Mee
Park Keun-Young
Huh Nam
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Abstract
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Background/Aims: The prevalence of diabetes in Korea is reported to be approximately 10%, but cases of maturity-onset diabetes of the young (MODY) are rare in Korea. A diagnostic technique for autosomal dominant MODY is being actively sought. In this regard, we used a DNA chip to investigate the frequency of mutations of the MODY3 gene (hepatocyte nuclear factor-1α) in Korean patients with early-onset type 2 diabetes.
Methods: The genomic DNA of 30 normal individuals [age, 24.9±8.6 years] and 25 patients with early-onset type 2 diabetes (age, 27±5.9 years) was extracted, and the MODY3 gene was amplified. The amplified DNA was hybridized onto a MODY3 chip, which has oligonucleotides of 15-25 bases, representing wild-type and mutant MODY3 sequences in both forward and reverse orientations, immobilized on its surface.
Results: Among the normal subjects, there was no mutation of MODY3. Among those with early-onset type 2 diabetes, there was one case of MODY3 mutation.
Conclusions: Our results indicate that MODY3 mutations are not rare in Korean early-onset type 2 diabetes patients in Korea and suggest that MODY3 mutations in patients with early-onset type 2 diabetes need to be further evaluated.
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KEYWORD
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DNA chips, MODY, Type 2 diabetes mellitus
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