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KMID : 0338420130280040420
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The Korean Journal of Internal Medicine
2013 Volume.28 No. 4 p.420 ~ p.427
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The dose of cyclophosphamide for treating paraquat-induced rat lung injury
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Choi Jae-Sung
Jou Sung-Shick
Oh Mee-Hye
Kim Young-Hee
Park Min-Ju
Gil Hyo-Wook
Song Ho-Yeon
Hong Sae-Yong
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Abstract
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Background/Aims: Cyclophosphamide (CP) is a promising treatment for severe cases of paraquat (PQ) poisoning. We investigated the effective dose of CP for mitigating PQ-induced lung injury.
Methods: Adult male Sprague-Dawley rats were allocated into five groups: control, PQ (35 mg/kg, intraperitoneal injection), and PQ + CP (1.5, 15, or 30 mg/kg). The dimensions of lung lesions were determined using X-ray microtomography (micro-CT), and histological changes and cytokine levels were recorded.
Results: The micro-CT results showed that 15 mg/kg CP was more effective than 1.5 mg/kg CP for treating PQ-induced lung injury. At a dose of 1.5 mg/kg, CP alleviated the histological evidence of inflammation and altered superoxide dismutase activity. Using 15 mg/kg CP reduced the elevated catalase activity and serum transforming growth factor (TGF)-β1 level.
Conclusions: A CP dose of > 15 mg/kg is effective for reducing the severity of PQ-induced lung injury as determined by histological and micro-CT tissue examination, possibly by modulating antioxidant enzyme and TGF-β1 levels.
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KEYWORD
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Paraquat, Cyclophosphamide, Reactive oxygen species, X-ray microtomography
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