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KMID : 0338420170320050883
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The Korean Journal of Internal Medicine
2017 Volume.32 No. 5 p.883 ~ p.889
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Caveolin-1 is involved in high glucose accelerated human glomerular mesangial cell senescence
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Feng Xin
Gao Wei
Li Yao
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Abstract
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Background/Aims: We demonstrated the role of caveolin-1 involved in high glucose (HG)-induced glomerular mesangial cells (GMCs) senescence.
Methods: HG was used to stimulate GMCs. The telomere lengths were analyzed by Southern blot. β-Galactosidase staining was determined. The expressions of caveolin-1 and P53 proteins were determined by Western blot.
Results: Treatment with high concentrations of glucose induced GMC senescence accompanied by shortened telomere length and increase of β-galactosidase staining as well as P53 protein, which was abrogated after application of caveolin-1-siRNA.
Conclusions: This study proved that HG induced cell senescence in GMCs. The caveolin-1 is involved in HG-induced mesangial cell senescence, and blocking caveolin-1 significantly reduced cell senescence. The effect of caveolin-1 is mediated by P53 pathway.
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KEYWORD
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Mesangial cells, Aging, Caveolin-1
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