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KMID : 0338420210360010164
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The Korean Journal of Internal Medicine
2021 Volume.36 No. 1 p.164 ~ p.174
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Usefulness of BK virus-specific interferon-γ enzyme-linked immunospot assay for predicting the outcome of BK virus infection in kidney transplant recipients
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Bae Hyun-Joo
Na Do-Hyun
Chang Ji-Yeun
Park Ki-Hyun
Min Ji-Won
Ko Eun-Jeong
Lee Hye-Young
Yang Chul-Woo
Chung Byung-Ha
Oh Eun-Jee
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Abstract
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Background/Aims: To investigate if BK virus (BKV)-specific T cell immunity measured by an interferon-γ enzyme-linked immunospot (ELISPOT) assay can predict the outcome of BK virus infection in kidney transplant recipients (KTRs).
Methods: We included 68 KTRs with different viremia status (no viremia [n = 17], BK viremia [n = 27], and cleared viremia [n = 24]) and 44 healthy controls (HCs). The BK viremia group was divided into controller (< 3 months) and noncontroller (> 3 months) according to sustained duration of BKV infection. We compared BKV-ELISPOT results against five BKV peptides (large tumor antigen [LT], St, VP1-3).
Results: BKV-ELISPOT results were higher in three KTRs groups with different BKV infection status than the HCs group (p < 0.05). In KTR groups, they were higher in cleared viremia group than no viremia or BK viremia group. Within the BK viremia group, controller group had higher LT-ELISPOT results compared to noncontroller group (p = 0.032). Also, KTRs without BK virus-associated nephropathy (BKVN) had higher LT, St, VP1, and VP2-ELISPOT results than those with BKVN (p < 0.05).
Conclusions: BKV-ELISPOT assay may be effective in predicting clinical outcomes of BKV infection in terms of clearance of BK virus and development of BKVN.
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KEYWORD
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BK virus, Kidney transplantation, Viremia, BK polyomavirus-associated nephropathy, Enzyme-linked immunospot assay
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