KMID : 0338420210360030596
|
|
The Korean Journal of Internal Medicine 2021 Volume.36 No. 3 p.596 ~ p.607
|
|
The relationship between miRNA-26b and connective tissue growth factor in rat models of aortic banding and debanding
|
|
Cho Jung-Sun
Lee Jong-Ho Park Ki-Cheol Yang Keum-Jin Cho Eun-Joo
|
|
Abstract
|
|
|
Background/Aims: Connective tissue growth factor (CTGF) is a profibrotic factor implicated in pressure overload-mediated myocardial fibrosis. In this study, we determined the role of predicted CTGF-targeting microRNAs (miRNAs) in rat models of aortic stenosis and reverse cardiac remodeling.
Methods: Minimally invasive ascending aortic banding was performed in 24 7-week-old male Sprague-Dawley rats, which were divided into three groups. The banding group consisted of eight rats that were sacrificed immediately after 6 weeks of aortic constriction. The debanding group underwent aortic constriction for 4 weeks and was sacrificed 2 weeks after band removal. The third group underwent sham surgery. We investigated the expression of CTGF, transforming growth factor-¥â1 (TGF¥â1), and matrix metalloproteinase-2 using ELISA and examined miRNA-26b, miRNA-133a, and miRNA-19b as predicted CTGF-targeting miRNAs based on miRNA databases in 24-hour TGF¥â-stimulated and TGF¥â- washed fibroblasts and myocardial tissues from all subjects.
Results: CTGF was elevated in 24-hour TGF¥â-stimulated fibroblasts and decreased in 24-hour TGF¥â-washed fibroblasts. miRNA-26b was significantly increased in TGF¥â-washed fibroblasts compared with control and TGF¥â-stimulated fibroblasts (p < 0.05). CTGF expression was significantly higher in the banding group than that in the sham and debanding groups. The relative expression levels of miRNA-26b were higher in the debanding group than in the banding group.
Conclusions: The results of our study using models of aortic banding and debanding suggested that miRNA-26b was significantly increased after aortic debanding. The in vitro model yielded the same results: miRNA-26b was upregulated after removal of TGF¥â from fibroblasts.
|
|
KEYWORD
|
|
Fibrosis, Aortic banding, Aortic debanding, Connective tissue growth factor, miRNA-26b
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|