|
KMID : 0352720190430020282
|
|
Journal of Ginseng Research
2019 Volume.43 No. 2 p.282 ~ p.290
|
|
|
Ginsenoside Rg3 suppresses mast cell?mediated allergic inflammation via mitogen-activated protein kinase signaling pathway
|
|
Kee Ji-Ye
Hong Seung-Heon
|
|
|
Abstract
|
|
|
Background: Ginsenoside Rg3 (G-Rg3) is the major bioactive ingredient of Panax ginseng and has many pharmacological effects, including antiadipogenic, antiviral, and anticancer effects. However, the effect of G-Rg3 on mast cell?mediated allergic inflammation has not been investigated.
Method: The antiallergic effects of G-Rg3 on allergic inflammation were evaluated using the human and rat mast cell lines HMC-1 and RBL-2H3. Antiallergic effects of G-Rg3 were detected by measuring cyclic adenosine monophosphate (cAMP), detecting calcium influx, and using real-time reverse transcription polymerase chain reaction, enzyme-linked immunosorbent assay, Western blotting, and in vivo experiments.
Results: G-Rg3 decreased histamine release from activated mast cells by enhancing cAMP levels and calcium influx. Proinflammatory cytokine production was suppressed by G-Rg3 treatment via regulation of the mitogen-activated protein kinases/nuclear factor-kappa B and receptor-interacting protein kinase 2 (RIP2)/caspase-1 signaling pathway in mast cells. Moreover, G-Rg3 protected mice against the IgE-mediated passive cutaneous anaphylaxis reaction and compound 48/80-induced anaphylactic shock.
Conclusion: G-Rg3 may serve as an alternative therapeutic agent for improving allergic inflammatory disorders.
|
|
|
KEYWORD
|
|
|
Allergic inflammation, Ginsenoside Rg3, Mast cells, Mitogen-activated protein kinase, Nuclear factor-κB
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|