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KMID : 0362420060440050574
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Journal of Korean Academy of Prosthodontics
2006 Volume.44 No. 5 p.574 ~ p.583
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THE EFFECT OF PERMANENT MAGNET CONNECTING WITH DENTAL IMPLANT ON BONE EXTRACELLULAR MATRIX FORMATION
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Won In-Jae
Lee Sung-Bok
Baik Jin
Kwon Kung-Rock
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Abstract
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Statement of problem: The use of permanent magnetics is increasing in implant dentistry.
Purpose: This study is to know the effect of permanent magnetics on bone matrix formation of osteoblasts.
Materials and methods: The konus abutment-shaped permanent magnetics were connected to the implant fixture, and placed on the culture plate. The osteoblast-like cell Mc3T3E1 were used for cell culture. As the control group, the implants were connected to titanium healing caps, and cultured in the same conditions of experimental group. After 3. 7, 14 days, cells were cultured, and we measured and compared the amount of collagen type I, osteocalcin, which is bone matrix protein by Western immunoblotting analysis.
Results: As a result of Western immunoblotting analysis for estimating the amount of bone extracellular matrix, there was no difference between osteoblast of the experimental group and the control group during 3 and 7day-osteoblast culturing. However when cells were cultured for 14days, the amount of bone extracellular matrix was increased, on the experimental group.
Conclusion: From these results, magnetic field of permanent magnetics might have effect on bone formation of osteoblast, especially at initial stage of implant placement. Therefore, their clinical application for implant or bone graft could be possible.
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KEYWORD
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Bone extracellular matrix, Permanent magnet, Collagen type I, Osteocalcin
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