KMID : 0364820090450020193
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Korean Journal of Microbiology 2009 Volume.45 No. 2 p.193 ~ p.199
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Isolation and Identification of Halotolerant Bacillus sp. SJ-10 and Characterization of Its Extracellular Protease
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Kim Eun-Young
Kim Dong-Gyun Kim Yu-Ri Choi Sun-Young Kong In-Soo
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Abstract
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A bacterium producing the halotolerant extracellular protease was isolated from squid jeotgal, and was identified as Bacillus sp. SJ-10 based on morphological, physiological and biochemical characteristics, as well as phylogenetic analysis using 16S rRNA gene sequence. The strain grew at 20oC~55oC, pH 5~8, and 0%~14% NaCl and optimal growth conditions were 35¡¾5oC, pH 7, and 5% NaCl. The major cellular fatty acids were anteiso-C15:0, anteiso-C17:0, and C16:0. DNA G+C content was 50.58 mol% and menaquinone consisted of MK-7. Phylogenic analysis based on the 16S rRNA gene sequence indicated that SJ-10T belongs to the genus Bacillus. About 40 kDa of the salt-tolerant protease was purified by 40% ammonium sulfate saturation and Mono Q column chromatography. The optimal activity of the protease was pH 8 and stable at pH 5~10. The optimum temperature and NaCl concentration were 35¡¾5oC and 5¡¾1%, respectively.
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KEYWORD
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Bacillus sp., collagen degrading enzyme, halophilic bacteria
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