Background : ischemia/reperfusion injury is very important issue in the era of thrombolysis and primary coronary angioplasty in acute myocardial infarction. However, the mechanism of ischemia-reperfusion injury is not fully clarified. Estrogen is well known to have protective actions against ischemic heart disease. We tested hypothesis that estrogen may protect myocardium by reducing ischemia/reperfusion-induced apoptosis. Methods : Sprague-Dawley rats that underwent ovariectomy, female controls, and male rats were subjected to 45minutes of left coronary artery occlusion followed by 4 or 24hours of reperfusion. Coronary artery occlusion was performed 1 week after ovariectomy or sham operation. And sham operation was also performed in each group to confirm the effects of ischemia/reperfusion. Results : Ischemia/reperfusion induced apoptosis in myocardium, especially at border zone, whereas sham operation did not induce apoptosis. After 4 hours of
x reperfusion the percentages of apoptotic myocytes in border and center zone of reperfused area were 35.7±3. 7%, 29.0±4.2% in ovariectomized rats (n=3), 40.8±3.7%, 29.5±2.5% in female control rats (n=3), and 39. 0±1.6% (p=0.10), 32.4±1.6% (p=0.43) in male rats (n=3). After 24hours of reperfusion the percentages in border and center zone of reperfused area were 20.6±3.1%,12.9±4.8% in ovariectomized rats (n=3),19.6± 4.1%, 14.0±2.0% in female control rat (n=3), and 21.4±6.6% (p=0.93), 15.1±2.4% (p=0.85) in male rats (n=3). There is difference of apoptosis neither between male and female rats nor between control and ovariectomized female rats. Conclusion : Estrogen did not affect the ischemia/reperfusion-induced apoptosis in rat myocardium. (Korean Circulation J 1999:29 (9):956-964)
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