|
KMID : 0368120070370020078
|
|
Korean Circulation Journal
2007 Volume.37 No. 2 p.78 ~ p.83
|
|
|
A Slight Variation in the Age of Rats Commonly used as a Carotid Artery Injury Model Results in a Large Difference in Neointima Formation
|
|
Kwon Jin-Sook
Park No-Kwan
Jeong Il-Ha
Kim Yu-Kyung
Bae Jang-Wahn
Hwang Kyung-Kuk
Kim Dong-Woon
Cho Myeong-Chan
|
|
|
Abstract
|
|
|
Background & Objectives: The degree of neointima formation after infliction of a carotid artery balloon injury in rats varies greatly depending on the sex, age, species and operational method. Strong variation is common, even within only a single control. This study attempted to find if there was any significant difference in neointima formation following a carotid artery balloon injury in 6 to 12 week old rats; the age commonly used in these types of experiments.
Materials & Methods: A balloon injury was inflicted on the carotid arteries of male SpragueDawley rats at 6 (n=9, 250-270 g), 8 (n=8, 280-300 g) and 11 weeks (n=10, 320-340 g) of age. Two weeks postoperation, a histomorphometric analysis was carried out. The vascular smooth muscle cell proliferation was measured in situ via BrdU incorporation 2 days after injury infliction.
Results: The neointima areas of the 6 week (0.22±0.04 mm2) and 8 week old groups (0.17±0.08 mm2) were 3.1 and 2.4 times larger than that of the 11 week old group (0.07±0.03 mm2). The mitotic index was significantly reduced in 11 week old group (n=4, 9.22±1.51%) compared to those of the 6 (n=4, 25.03±3.92%) and 8 week old (n=4, 21.66±3.66%) groups.
Conclusion: Special care should be taken when interpreting neointima formation, as even a slight variation in the age and weight in 6 to 12 week old (250-340 g) rats; the age commonly used in these types of experiments, results in an unexpectedly large difference.
|
|
|
KEYWORD
|
|
|
Neointimal formation, Carotid artery injuries, Aging
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
   
|
|