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KMID : 0368120070370070327
Korean Circulation Journal
2007 Volume.37 No. 7 p.327 ~ p.333
Protection of Cardiomyocytes from Acute Ischemic Injury by Protein Kinase C¥å Expression
Yoo Jeong-Nam

Lee Soo-Hoon
Jang Sun-Ik
Kim Sang-Ok
Lee Kwang-Hee
Park Min-A
Lim Tae-Hyung
Kwon Jin-Sook
Cho Myeong-Chan
Kim Young-Dae
Abstract
Background and Objectives£ºIschemic injury is the most common and important cause of myocardial damage. Over past decades, a number of studies have identified a protective mechanism known as ischemic preconditioning, which can block or delay cell death from ischemic injury. Protein kinase C (PKC), especially the¥åisoform has been proposed as a key factor in the signaling pathway of ischemic preconditioning. However, whether PKC¥å expression in cardiomyocytes can offer such protection from acute ischemia has not been explored.

Materials and Methods£ºTo demonstrate a direct effect of PKC¥å expression, a lentiviral vector system was established. Using the lentiviral vector, PKC¥å was introduced to neonatal rat ventricular myocytes (NRVM) cultured under ischemic conditions, and also to adult rat myocardium subject to left coronary artery ligation.

Results£ºCompared to control, PKC¥å expression in cultured NRVM under ischemia resulted in preserved cell density and morphology, and a reduction in cell death (77.6¡¾12.8% vs 58.1¡¾7.2%, p<0.05). In adult rats, the infarcted area after coronary artery ligation was markedly reduced in myocardium injected with PKC¥å vector compared to control (11.4¡¾5.3% vs 20.5¡¾11.3%, p<0.01).

Conclusion£ºThese results provide direct evidence that PKC¥å is a central player in protection against cell death from acute ischemia.
KEYWORD
Myocytes, cardiac, Protein kinase C-epsilon, Lentivirus, Ischemic preconditioning
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