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KMID : 0368120150450030234
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Korean Circulation Journal
2015 Volume.45 No. 3 p.234 ~ p.241
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Glucose-Insulin-Potassium Solution Protects Ventricular Myocytes of Neonatal Rat in an In Vitro Coverslip Ischemia/Reperfusion Model
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Chun Woo-Jung
Nah Deuk-Young
Bae Jun-Ho
Chung Jin-Wook
Lee Hyun-Sook
Moon Il-Soo
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Abstract
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Background and Objectives: The benefit of high glucose-insulin-potassium (GIK) solution in clinical applications is controversial. We established a neonatal rat ventricular myocyte (NRVM) in vitro coverslip ischemia/reperfusion (I/R) model and investigated the effects of GIK solution on suppressing reactive oxygen species (ROS) and upregulating O-GlcNacylation, which protects cells from ischemic injury.
Materials and Methods: NRVMs were isolated from postnatal day 3-4 Sprague-Dawley rat pups and grown in Dulbecco's modified Eagle's medium containing high glucose (4.5 g/L), fetal bovine serum, and penicillin/streptomycin. The effects of the GIK solution on ROS production, apoptosis, and expression of O-GlcNAc and O-GlcNAc transferase (OGT) were investigated in the coverslip I/R model.
Results: Covering the 24-well culture plates for 3 hr with 12 mm diameter coverslips resulted in the appropriate ischemic shock. Glucose and insulin synergistically reduced ROS production, protected NRVM dose-dependently from apoptosis, and altered O-GlcNAc and OGT expression.
Conclusion: The high GIK solution protected NRVM from I/R injury in vitro by reducing ROS and altering O-GlcNacylation.
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KEYWORD
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Myocytes, cardiac, Glucose-insulin-potassium cardioplegic solution, GlcNAc, O-GlcNac transferase
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