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KMID : 0368120200500030236
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Korean Circulation Journal
2020 Volume.50 No. 3 p.236 ~ p.247
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Novel Associations between Related Proteins and Cellular Effects of High-Density Lipoprotein
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Choi Seung-Bum
Park Yae-Eun
Cheon Eun-Jeong
Kim Kyeong-Yeon
Kim Mi-So
Ann Soo-Jin
Noh Hye-Min
Lee Jae-Ho
Lee Chan-Joo
Lee Seung-Taek
Lee Cheol-Ju
Lee Ji-Eun
Lee Sang-Hak
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Abstract
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Background and Objectives: Recent studies have examined the structure-function relationship of high-density lipoprotein (HDL). This study aimed to identify and rank HDL-associated proteins involved in several biological function of HDL.
Methods: HDLs isolated from 48 participants were analyzed. Cholesterol efflux capacity, effect of HDL on nitric oxide production, and vascular cell adhesion molecule-1 expression were assessed. The relative abundance of identified proteins in the highest vs. lowest quartile was expressed using the normalized spectral abundance factor ratio.
Results: After adjustment by multiple testing, six proteins, thyroxine-binding globulin, alpha-1B-glycoprotein, plasma serine protease inhibitor, vitronectin, angiotensinogen, and serum amyloid A-4, were more abundant (relative abundance ratio ≥2) in HDLs with the highest cholesterol efflux capacity. In contrast, three proteins, complement C4-A, alpha-2-macroglobulin, and immunoglobulin mu chain C region, were less abundant (relative abundance ratio <0.5). In terms of nitric oxide production and vascular cell adhesion molecule-1 expression, no proteins showed abundance ratios ≥2 or <0.5 after adjustment. Proteins correlated with the functional parameters of HDL belonged to diverse biological categories.
Conclusions: In summary, this study ranked proteins showing higher or lower abundance in HDLs with high functional capacities and newly identified multiple proteins linked to cholesterol efflux capacity.
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KEYWORD
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Atherosclerosis, Cardiovascular diseases, Proteomics, Lipoproteins, Immunity
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