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KMID : 0368420130560010049
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Journal of Plant Biology
2013 Volume.56 No. 1 p.49 ~ p.58
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Mutations in AP22.65 accelerate flowering in Arabidopsis thaliana
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Xing Ji Hong
Wang Feng Ru
Jia Jiao
Zhang Jing
Li Li
Chen Zhan
Weng Qiao Yun
Yang Ping
Zhang Ye
Zhao Bin
Zhao Bin
Si He Long
Dong Jin Gao
Han Jian Min
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Abstract
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Identification of the gene(s) responsible for flowering time in Arabidopsis has significant implications. We used the T-DNA insertion library of Arabidopsis thaliana to screen an early-flowering mutant that exhibits accelerated flowering under short-day conditions. AP22.65, a novel flowering-time gene in that species, was isolated and identified via genome-walking and bioinformatics analysis. The flowering time of AP22.65-complementing plants was similar to that of the Col-0 wild type (WT). Conversely, its overexpression delayed flowering. Consistent with this phenotype, expression of AP22.65 was decreased in the ap22.65-1 mutant, recovered in AP22.65-complementing plants, and increased in AP22.65-overexpressing plants. Compared with the WT, expression levels of critical genes in different flowering pathways, i.e., SPY, FLC, GI, CO, FT, and LFY, were down-regulated in loss-of-function mutants. Expression of AP22.65 was distributed in flowers, siliques, rosette leaves, and whole seedlings. Therefore, this gene may be a negative regulator of Arabidopsis flowering.
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KEYWORD
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Arabidopsis thaliana, AP22.65, Early flowering
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