KMID : 0368420160590060616
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Journal of Plant Biology 2016 Volume.59 No. 6 p.616 ~ p.626
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Enhanced resistance of PsbS-deficient rice (Oryza sativa L.) to fungal and bacterial pathogens
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Zulfugarov Ismayil S.
Tovuu Altanzaya Kim Chi-Yeol Vo Kieu Thi Xuan Ko Soo-Yeon Hall Michael Seok Hye-Yeon Kim Yeon-Ki Skogstrom Oscar Moon Yong-Hwan Jansson Stefan Jeon Jong-Seong Lee Choon-Hwan
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Abstract
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The 22-kDa PsbS protein of Photosystem II is involved in nonphotochemical quenching (NPQ) of chlorophyll fluorescence. Genome-wide analysis of the expression pattern in PsbS knockout (KO) rice plants showed that a lack of this protein led to changes in the transcript levels of 406 genes, presumably a result of superoxide produced in the chloroplasts. The top Gene Ontology categories, in which expression was the most differential, included ¡®Immune response¡¯, ¡®Response to jasmonic acid¡¯, and ¡®MAPK cascade¡¯. From those genes, we randomly selected nine that were up-regulated. Our microarray results were confirmed by quantitative RT-PCR analysis. The KO and PsbS RNAi (knockdown) plants were more resistant to pathogens Magnaporthe oryzae PO6-6 and Xanthomonas oryzae pv. oryzae than either the wild-type plants or PsbS-overexpressing transgenic line. These findings suggest that superoxide production might be the reason that these plants have greater pathogen resistance to fungal and bacterial pathogens in the absence of energy-dependent NPQ. For example, a high level of cell wall lignification in the KO mutants was possibly due to enhanced superoxide production. Our data indicate that certain abiotic stress-induced reactive oxygen species can promote specific signaling pathways, which then activate a defense mechanism against biotic stress in PsbS-KO rice plants.
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KEYWORD
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Biotic stress tolerance, Microarray, Nonphotochemical quenching, PsbS, Rice, Superoxide
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