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KMID : 0376219870240030407
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Chonnam Medical Journal
1987 Volume.24 No. 3 p.407 ~ p.416
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Usefulness of Urinary Ribonucleosides as Markers for Cancer
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李民化/Lee, Min-wha
安鳳煥/梁誠烈/鄭榮鍍/金成烈/尹鐘晩/Ahn, Bong-whan/Yang, Sung-yenl/Choung, Young-doh/Oh, Sung-tack/Kim, Seong-ryui
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Abstract
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The usefulness of several breakdown products of tRNA in urine was assessed in cancer (gastric cancer, esophageal cancer and hepatoma) and in diseases other than cancer (gastric ulcer and chronic hepatitis). Separation and quantitation of urinary ribonucleosides were accomplished by using high-performance liquid chromatography (HPLC), and the excretion levels of urinary nucleosides were expressed as a function of urine cretonne concentration.
Of 15 urinary ribonucleosides which could be separated by HPLC, pseudouridine level was most predominantly elevated in cancer. As compared to normal levels (19.55±4.44 nmoles/μmole creatinine, n=14), the patients with gastric cancer (47.99±20.92, n=20), esophageal cancer (42.13±16.69, n=5) and hepatoma (35.29±3.67, n=4) showed approximately two-fold increase in the excretion of pseudouridine (p<0.01). All of the cancer patients -also showed pseudouridine elevations over the value of normal mean plus 2 . standard deviations. Although urinary pseudouridine levels were also significantly elevated in non-cancer patients (gastric ulcer, 40.54±21.45, n=13, and chronic hepatitis, 33.67±17.06, n=6), only 53% of the patients showed the elevation over the value of {formal mean plus 2 standard deviations.
The urinary excretion levels of 1-methylinosine and 1-methyladenosine were also significantly elevated in gastric cancer patients, and their elevations over the value of normal mean plus 2 standard deviations were found in 64~71% of the patients. In contrast, none of gastric ulcer and hepatitis patients showed significant elevations of urinary 1-methylinosine and 1-me-thyladenosine levels.
The follow-up study in several patients undergone surgery showed that elevated levels of the urinary nucleosides approached normal levels on the 12th day after surgery.
These results indicate that since urinary pseudouridine level is significantly elevated not only in cancer patients but also in patients with diseases other than cancer, determination of more than one nucleoside levels is more informative for the diagnosis of cancer than the determination of single pseudouridine level.
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