KMID : 0379520090250040175
|
|
Çѱ¹µ¶¼ºÇÐȸÁö 2009 Volume.25 No. 4 p.175 ~ p.180
|
|
Effect of Lead(¥³) Acetate on Procoagulant Activity in Human Red Blood Cells
|
|
Kim Keun-Young
Lim Kyung-Min Shin Jung-Hun Noh Ji-Yoon Ahn Jae-Bum Lee Da-Hye Chung Jin-Ho
|
|
Abstract
|
|
|
Lead (Pb) is a ubiquitously occurring environmental heavy metal which is widely used in industry and human life. Possibly due to a global industrial expansion, recent studies have revealed the prevalent human exposure to Pb and increased risk of Pb toxicity. Once ingested by human, 95% of absorbed Pb is accumulated into erythrocytes and erythrocytes are known to be a prime target for Pb toxicity. Most of the studies were however, focused on Pb©÷? whereas the effects of Pb©ù?, another major form of Pb on erythrocytes are poorly understood yet. In this study, we investigated and compared the effects of Pb©ù?, Pb©÷? and other heavy metals on procoagulant activation of erythrocytes, an important factor for the participation of erythrocytes in thrombotic events in an effort to address the cardiovascular toxicity of Pb©ù?. Freshly isolated erythrocytes from human were incubated with Pb©ù?, Pb©÷?, Cd©÷? and Ag? and the exposure of phosphatidylserine (PS), key marker for procoagulant activation was measured using flow cytometry. As a result, while Cd©÷? and Ag? did not affect PS exposure, Pb©ù? and Pb©÷? induced significantly PS exposure in a dose-dependent manner. Of a particular note, Pb©ù? induced PS exposure with a similar potency with Pb©÷?. PS bearing microvesicle (MV), another important contributor to procoagulant activation was also generated by Pb©ù?. These PS exposure and MV generation by Pb©ù? were well in line with the shape change of erythrocyte from normal discocytes to MV shedding echinocytes following Pb©ù? treatment. Meanwhile, nonspecific hemolysis was not observed suggesting the specificity of Pb©ù?-induced PS exposure and MV generation. These results indicated that Pb©ù? could induce procoagulant activation of erythrocytes through PS exposure and MV generation, suggesting that Pb©ù? exposure might ultimately lead to increased thrombotic events.
|
|
KEYWORD
|
|
Lead, Pb©ù?, Red blood cell, Phosphatidylserine exposure, Microvesicle generation, Hemolysis
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|