KMID : 0379520160320040311
|
|
Çѱ¹µ¶¼ºÇÐȸÁö 2016 Volume.32 No. 4 p.311 ~ p.316
|
|
Skin Corrosion and Irritation Test of Nanoparticles Using Reconstructed Three-Dimensional Human Skin Model, EpiDermTM
|
|
Kim Hye-Jin
Choi Jong-Hye Lee Han-Dule Park Ju-Young Yoon Byung-Il Jin Seon-Mi Park Kwang-Sik
|
|
Abstract
|
|
|
Effects of nanoparticles (NPs) on skin corrosion and irritation using three-dimensional human skin models were investigated based on the test guidelines of Organization for Economic Co-operation and Development (OECD TG431 and TG439). EpiDermTM skin was incubated with NPs including those harboring iron (FeNPs), aluminum oxide (AlNPs), titanium oxide (TNPs), and silver (AgNPs) for a defined time according to the test guidelines. Cell viabilities of EpiDermTM skins were measured by the 3-(4, 5-dimethylthi-azol-2-yl)-2.5-diphenyltetrazolium bromide based method. FeNPs, AlNPs, TNPs, and AgNPs were non-corrosive because the viability was more than 50% after 3 min exposure and more than 15% after 60 min exposure, which are the non-corrosive criteria. All NPs were also non-irritants, based on viability exceeding 50% after 60 min exposure and 42 hr post-incubation. Release of interleukin 1-alpha and histopathological analysis supported the cell viability results. These findings suggest that FeNPs, AlNPs, TNPs, and AgNPs are ¡®non-corrosive¡¯ and ¡®non-irritant¡¯ to human skin by a globally harmonized classification system.
|
|
KEYWORD
|
|
Nanoparticles, Skin model, Alternative methods, Skin corrosion, Skin irritation
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|