KMID : 0379520210370010071
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Çѱ¹µ¶¼ºÇÐȸÁö 2021 Volume.37 No. 1 p.71 ~ p.83
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Beneficial effects of Diplectria barbata (Wall. Ex C. B. Clarke) Franken et Roos extract on aging and antioxidants in vitro and in vivo
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Hong Young-Eun
Lee Hyun-Ji Tran Quangdon Bayarmunkh Choinyam Boldbaatar Damdindorj Kwon So-Hee Park Jong-Sun Park Ji-Soo
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Abstract
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The purpose of this study is to explore the effects of Diplectria barbata (Wall. Ex C.B. Clarke) Franken & Roons (DFR) on wound healing, antioxidant and aging in Normal Human Dermal Fibroblast cell (NHDF) cells and mouse skin models. We investigated the effects of the aging process in vitro and in vivo. DFRtreated NHDF cells showed a concentration-dependent increase in the expression of extracellular matrix (ECM) proteins (Collagen-2.5-fold increase at 50 ¥ìg/ml, Elastin-1.5-fold increase at 1¥ìg/ml) as well as an increase in proteins related to cell survival, differentiation, and development, while expression of aging proteins such as matrix metalloproteinase 3 (MMP-3) was decreased (5-fold decrease at 50 ¥ìg/ml). DFR treatment also led to enhanced expression of antioxidant proteins such as nuclear factor erythroid 2-related factor 2 (10-fold increase at 50 ¥ìg/ml) and heme oxygenase 1 (1.5-fold increase at 25 ¥ìg/ml). To further investigate the antioxidative effects of DFR extracts, the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activities were also evaluated. DFR extracts improved wound healing and resulted in increased expression of ECM proteins, while enzymes involved in collagen degradation, including MMP-3, were decreased in NHDF cells as well as in a mouse model. This study demonstrates the anti-aging, antioxidant, and wound healing properties of DFR extracts. Therefore, DFR extracts present may facilitate skin protection and care.
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KEYWORD
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Anti-aging, Anti-oxidant, Wound healing, Diplectria barbata (Wall. Ex C.B. Clarke) Franken & Roos, Extracts, NHDF cells
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