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KMID : 0380619910230050561
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Korean Journal of Food Science and Technology
1991 Volume.23 No. 5 p.561 ~ p.567
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L - Methionine Production by Protoplast Fusion of Brevibacterium flavum ATCC 14067 and Corynebacterium glutamicum ATCC 13032
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Abstract
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This study was designed to investigate the productivity of L-methionine by the method of protoplastfusion between Brevibacterium flavum ATCC 14067 and Corynebacterium glutamicm ATCC 13032, and then L-methionine production was performed to continuous fermentation using the immobilized fusant cells. Mutants B. flavum K 104(thr met Km^r Et Sm Tm^r as genetic marker) and C. glutamicum B 70(thr^- hos^- Km^r Et^r Sm^- Tm^r as genetic marker) were isolated by MNNG treatment. On the other hand, protoplast of mutants were formed to treat with lysis solution containing 500 μg/ml of lysozyme. The ratios of protoplast formation and regeneration were 99% and 64∼66% respectively. Fusion frequency between B. flavum K 104 and C. glutamicum B 70 showed the 3.5 X 10^5 in the 35% polyethylene glycol(PEG6000) containing 3% PVP solution. The productivity of L-methionine by fusant BFCG 37 immobilized with sodium alginate was 0.89 g/l the batch fermentation and was 18.75 mgl^(-1)h^(-1) on the continuous fermentation at 30 ℃ for 72 hr.
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