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KMID : 0380620010330030361
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Korean Journal of Food Science and Technology
2001 Volume.33 No. 3 p.361 ~ p.365
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The Regulation of Uric Acid on the Biosynthesis of Serratia marcescens and Lactobacillus plantarum Purine Nucleoside Phosphorylase
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Choi Byung-Bum
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Abstract
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The effects of purine catabolites in growth media on the biosynthesis of Serratia marcescens and Lactobacillus plantarum purine nucleoside phosphorylase (PNP) activity were examined. Serratia PNP activity was decreased approximately by 30% in the presence of high concentrations of inosine (5∼15 mM), but was not affected at low concentrations of inosine (0.1∼1 mM). However, Lactobacillus PNP activity was increased above 60% by inosine among the range from 5 to 15 mM. Serratia PNP activity was decreased approximately by 45% in the presence of high concentrations of hypoxanthine (5∼15 mM), but was not affected at low concentrations of hypoxanthine (0.1∼0.5 mM). Lactobacillus PNP activity was increased approximately by 20% in the presence of low concentrations of hypoxanthine (0.1∼0.5 mM), and increased approximately by 50∼65% in the presence of concentrations of hypoxanthine (1∼15 mM). Serratia and Lactobacillus PNP activity was increased 20% by low concentrations of uric acid (0.5 mM), but was decreased 40∼80% at high concentrations of same purine catabolite (10∼15 mM). These data suggest that purine nucleoside phosphorylase in Serratia marcescens ATCC 25419 and Lactobacillus plantarum ATCC 8014 is positively regulated by a low uric acid concentration, and then may play a regulatory role in a purine nucleotide catabolic pathway.
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