KMID : 0380620130450060801
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Korean Journal of Food Science and Technology 2013 Volume.45 No. 6 p.801 ~ p.804
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Production of Glutathione by the Yeast Mutant Saccharomyces cerevisiae Sa59
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Jang Hye-Yoon
Oh Chul-Hwan Oh Nam-Soon
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Abstract
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The glutathione contents of the selected mutants were investigated and found to be 6.1-15.8 mg/g-DCW. The glutathione content positively correlated with the antioxidant activity of the mutant strains (R2=0.488). Furthermore, the glutathione content of the mutant S. cerevisiae Sa-59 was approximately 38% greater than that of the wild type strain and, therefore, this mutant strain was selected for glutathione production. The volumetric glutathione content in a shaking culture was increased by about 70% compared to the static culture. In addition, the specific glutathione content was increased by ~19%. The volumetric glutathione content and specific glutathione content were increased by approximately 16% and 66%, respectively, when 0.04% glutamate, 0.04% cysteine and 0.04% glycine were added. Furthermore, the highest antioxidant activity was 0.52 as absorbance unit at 700 nm.
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KEYWORD
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glutathione, yeast mutant, Saccharomyces cerevisiae, antioxidant activity
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