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KMID : 0380620160480030289
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Korean Journal of Food Science and Technology
2016 Volume.48 No. 3 p.289 ~ p.295
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Chemical Characteristics and Immunostimulating Activity of Crude Polysaccharide Isolated from Commercial Instant Coffee
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Kwak Bong-Shin
Shin Kwang-Soon
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Abstract
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To elucidate the new biologically active ingredient in commercial instant coffee, a crude polysaccharide (ICP-0) was isolated by ethanol precipitation, and its immunostimulatory activity was estimated. ICP-0 mainly consisted of galactose (55.5%), mannose (25.7%), arabinose (6.0%), and galacturonic acid (10.1%), suggesting the possibility of its existence as a mixture of galactomannan or pectic polysaccharide. ICP-0 showed proliferative activity in peritoneal macrophages and splenocytes. ICP-0 dose-dependently augmented the production of nitric oxide and reactive oxygen species by peritoneal macrophages. In addition, murine peritoneal macrophages stimulated by ICP-0 showed enhanced production of various cytokines (tumor necrosis factor-α, interleukin-6, and interleukin-12) as compared to unstimulated murine peritoneal macrophages. In an in vitro assay for assessing intestinal immunomodulation, the ICP-0-treated Peyer’s patch cells showed higher bone marrow cell proliferation activity at 100 μg/mL and higher production of granulocyte-macrophage colony-stimulating factor, compared to the untreated Peyer’s patch cells. These results suggest that polysaccharides in commercial instant coffee have a potentiality for macrophage functions and the intestinal immune system.
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KEYWORD
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instant coffee, polysaccharide, immunostimulating activity, Peyer’s patch, intestinal immune system
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