KMID : 0381120080300020133
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Genes and Genomics 2008 Volume.30 No. 2 p.133 ~ p.140
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The Production and Cytological Analysis of Brassica napus -B Genome Chromosome Monosomic Addition Lines and Their Hybrids
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Li Maoteng
Jun Xiang Liu Jianmin Yu Longjiang Li Dianrong
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Abstract
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The Brassica napus -B genome monosomic addition lines (MALs) (AACC + B¡¯, 2n = 39) were developed from self-pollination of pentaploid hybrids (AABCC) that were derived from hybridization between hexaploid hybrids (AABBCC) and B. napus (AACC). The alien chromosomes of the B genome in MALs were identified by the GISH technique, by observation of the meiotic behavior of pollen mother cells (PMCs), and by B-genome-specific molecular marker analysis. Studies of the meiotic behavior of B. napus -B genome chromosome MALs at diakinesis revealed that the majority of the chromosome configuration was 19II+1I, which indicated that the alien B genome chromosome remained univalent in most cases. The laggard-free PMCs also appeared at a lower ratio, which indicated that the B genome chromosome could be transmitted into gametes. The chromosome configurations of 20II and 19II+2I that appeared in double MALs (AACC+ 2 chromosomes of the B genome) indicated different homoeology between different B genome chromosomes. The paired B genome bivalent in double MALs can be normally segregated at anaphase in most cases. PMCs with multivalents were observed in all the double MAL combinations, which indicated homology of the B genome chromosomes with the A or C genome chromosomes.
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KEYWORD
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Brassica napus -B genome, meiotic analysis, monosomic addition lines (MALs), double monosomic addition lines, SSR
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