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KMID : 0381120200420030283
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Genes and Genomics
2020 Volume.42 No. 3 p.283 ~ p.290
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Comparative analysis of bovine maternal corpus luteum microRNAs with aberrant and normal developed cloned fetus at late gestation
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Su Xiaohua
Wang Shenyuan
Gao Guangqi
Zhou Xinyu
Han Lidong
Su Guanghua
Zhang Jiaqi
Bai Wanfu
Wang Xiuying
Li Guangpeng
Zhang Li
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Abstract
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Background: The development efficiency of cloned cattle is extremely low (
Objective: Our aim was to explore this phenomenon by microRNAs expression profile analysis of maternal corpus luteum (CL), which was related to pregnancy maintenance.
Methods: The present study provided the comparison of maternal CL miRNAs expression of abnormally and normally developed cloned bovine fetus at late gestation (~?210 days) using RNA-Seq technology.
Results: We selected two abnormally pregnant cows (abnormal group, AG) and three normally pregnant cows (normal group, NG) and acquired valid reads of 9317,261?12,327,185 (~?84.53?91.28%) from five libraries. In total, we identified 981 conserved miRNAs and 223 novel miRNAs. 1052 miRNAs were co-expressed, 124 miRNAs were uniquely expressed in AG, and 93 miRNAs were uniquely expressed in the NG. Compared with NG, 11 were significantly overexpressed, and 22 were downregulated (p
Conclusion: Our research suggested that abnormal miRNAs expression of bovine maternal CL may affect the pregnant status at late gestation.
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KEYWORD
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Corpus luteum, miRNA, Cloned bovine development, Abortion
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