KMID : 0381120200420111281
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Genes and Genomics 2020 Volume.42 No. 11 p.1281 ~ p.1290
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Characterization and functional analysis of Krtap11-1 during hair follicle development in Angora rabbits (Oryctolagus cuniculus)
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Liang Shuang
Bao Zhiyuan Zhao Bohao Zhou Tong Li Jiali Liu Ming Hu Shuaishuai Yang Naisu Chen Yang Wu Xinsheng
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Abstract
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Background: Keratin-associated protein (KAP), the structural protein molecule of hair fibers, plays a key role in determining the physical properties of hair. Studies of Krtap11-1 have focused only on its localization. Functional studies of Krtap11-1 in hair follicle development have so far not been reported.
Objective: This study aimed to provide evidence for the role of Krtap11-1 in skin and hair development.
Methods: Full-length cloning and analysis of Krtap11-1 were conducted to ascertain its function. Overexpression vectors and interference sequences were constructed and transfected into RAB-9 cells. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to investigate the hair follicle developmental stage of Krtap11-1, the expression of different tissues, and the effects on other hair follicle development-related genes.
Results: The full length of cloned Krtap11-1 was 947 bp. Krtap11-1 was confirmed to be a hydrophilic protein localized mostly in mitochondria. The greatest mRNA expression was observed in skin. Using a follicle synchronization model, it was found that Krtap11-1 mRNA expression levels first increased then decreased over the passage of time, principally during hair follicle catagen and telogen. Following the overexpression of Krtap11-1, mRNA expression levels of the WNT-2, KRT17, BMP-2, and TGF-¥â-1 genes increased, and LEF-1 decreased (P?0.05), the converse after the corresponding use of si-RNA interference.
Conclusions: Krtap11-1 exerts a promoting effect. The results provide novel insight into the relationship between hair follicle development and Krtap11-1 gene expression.
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KEYWORD
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Krtap11-1, Angora rabbit, Hair follicle development, Expression analysis
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